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Perkin elmer cetus geneamp pcr dna thermal cycler 9600
PERKIN ELMER CETUS GENEAMP PCR DNA THERMAL CYCLER 9600
SearchBy1 - 16287 | Location: FG1 | By: Matty | Tester: Chino | Quan: 1 | List: 1 | Duplicate? False | Plan: D
Standard Relist: True | Size: 26x30x18 Weight: 90 | MGR: Octavio | Start: $199.99 | BIN: $164.99 | Type: Fixed Price
PERKIN ELMER CETUS GENEAMP PCR SYSTEM 9600
The GeneAmp PCR System 9600 automates the Polymerase Chain Reaction (PCR) technique for amplifying DNA. The Polymerase Chain Reaction (PCR) technique is conceptually a very simple method for amplifying nucleic acids. It mimics the natural DNA replication process in that the number of DNA molecules generated by the Polymerase Chain Reaction doubles after each cycle. In the PCR technique, the typical cycle consists of three steps: Denaturing the template DNA by heating it to a high temperature (94 degrees C to 95 degrees C), thereby producing two single strands of DNA. Annealing of the target-specific primers to the two separated DNA strands by cooling the reaction mixture to a lower temperature (37 degrees C to 65 degrees C). Extending the annealed primers with a DNA polymerase by warming the reaction mixture to an intermediate temperature (72 degrees C). The anneal and extend steps can be combined into a single setpoint, called two-temperature PCR. The original DNA segment can be either a small, discrete molecule whose sequence is already known or a part of a much larger molecule in a complex mixture, such as a chromosome fragment. The product of the reaction will be discrete double-stranded DNA molecules whose termini will be determined by the set of primers used. Because the copy number of the target DNA doubles after each cycle, amplification through 25 cycles would theoretically yield a 33 million fold increase of specific product. The PCR method readily lends itself to research applications. Cloning of the amplified sequence can be facilitated by extending the 5" end of the primers with short sequences harboring 1-3 restriction sites. Dideoxy sequencing can also be performed on the amplified product.
Dimensions: 26x30x18 inches - Weight: 90 pounds.
